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BAC Water

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Bacteriostatic Water — Sterile Diluent for Peptide Reconstitution

Bacteriostatic water is a sterile, non-pyrogenic water preparation that contains 0.9% benzyl alcohol as a bacteriostatic preservative. It is the standard diluent used to reconstitute lyophilized peptides prior to study use. The benzyl alcohol preservative is what lets the same vial be punctured multiple times over a ~28-day window without microbial growth – that is the difference between bacteriostatic water and plain sterile water for injection, and it is why bacteriostatic water is the default choice for multi-dose research peptide vials.


Why Bacteriostatic Water Is the Standard for Peptide Reconstitution

Lyophilized peptides arrive as a dry powder and must be dissolved before use. Using plain sterile water works for a single-use draw but provides no preservation against microbial contamination when the stopper is punctured repeatedly. Bacteriostatic water’s 0.9% benzyl alcohol content inhibits bacterial growth at room temperature and under refrigeration, which is why every multi-dose research peptide protocol specifies it.

Component What it does
Sterile water (USP) Dissolves lyophilized peptide; non-pyrogenic
Benzyl alcohol 0.9% Bacteriostatic preservative – prevents microbial growth after first puncture

Bacteriostatic Water vs Sterile Water

The two are frequently confused. Both are sterile at the time of manufacture; only bacteriostatic water contains a preservative.

Type Preservative Use pattern
Bacteriostatic water 0.9% benzyl alcohol Multi-dose; good for ~28 days after first puncture
Sterile water (SWFI) None Single-use only; discard after opening

Available Sizes

Format Sterile liquid, multi-dose vial
Composition Sterile water for injection + 0.9% benzyl alcohol
Aliasnamen BAC water, bacteriostatic water for injection, BWFI
Verfügbare Größen 2mL · 3mL · 10mL
Lagerung Room temperature unopened; 2-8°C recommended after first puncture
Verwendung Nur für Forschungszwecke – nicht zur Anwendung am Menschen

How to Use Bacteriostatic Water for Peptide Reconstitution

Apply the standard reconstitution formula:

Total mg in the peptide vial ÷ mL of bacteriostatic water added = mg per mL

  • A 5mg peptide vial + 2mL of bacteriostatic water = 2.5mg per mL
  • A 10mg peptide vial + 2mL of bacteriostatic water = 5mg per mL
  • A 10mg peptide vial + 1mL of bacteriostatic water = 10mg per mL

Inject the bacteriostatic water slowly against the side of the vial rather than directly onto the lyophilized powder. Swirl gently until fully dissolved – do not shake. Once reconstituted, store at 2-8°C.


Common Questions About Bacteriostatic Water

What is bacteriostatic water?

Bacteriostatic water is sterile water containing 0.9% benzyl alcohol as a preservative. The benzyl alcohol inhibits microbial growth after the vial stopper is first punctured, which is why it is used as a diluent for multi-dose research peptide vials.

What is the difference between bacteriostatic water and sterile water?

Both are sterile at the time of manufacture. The difference is the preservative. Bacteriostatic water contains 0.9% benzyl alcohol and can be punctured multiple times over ~28 days. Sterile water (SWFI) contains no preservative and is intended for single use – it should be discarded after opening.

How long is bacteriostatic water good for after opening?

Standard manufacturer guidance is 28 days after first puncture when stored between 2-8°C. That is also the window typically cited for reconstituted peptide solutions stored in bacteriostatic water.

Does bacteriostatic water need to be refrigerated?

Unopened vials are stable at room temperature. Once the stopper has been punctured, refrigeration at 2-8°C is recommended to extend the usable window.

Is bacteriostatic water FDA approved?

Bacteriostatic water is a pharmaceutical diluent regulated by the FDA as a prescription sterile water preparation. Every vial WWP ships is labeled and sold strictly for laboratory and research use only.


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