TB-4 (Thymosin Beta-4)

Volume Pricing

Price per pack (10 vials). Discount applies to this compound only – no mix and match.

TB-500 — Thymosin Beta-4 Actin-Binding Fragment with Systemic Tissue Repair Activity

TB-500 is a synthetic peptide fragment corresponding to the actin-binding domain of thymosin beta-4 (Tβ4), specifically the 17-amino acid sequence Ac-LKKTETQ-EKNTLSEK-LKKTET (sometimes described as the LKKTETQ core motif). Thymosin beta-4 itself was isolated by Allan Goldstein and colleagues at George Washington University in the 1960s as a component of thymic extracts, initially characterized for lymphocyte maturation activity. Subsequent work established Tβ4 as the most abundant intracellular actin-sequestering protein in most mammalian cell types, regulating the ratio of G-actin (monomeric) to F-actin (filamentous) through its WH2 (Wiskott-Homology 2) domain. The TB-500 fragment retains this actin-sequestration activity and adds a systemic distribution profile — unlike BPC-157, whose research is predominantly localized to the gastrointestinal system, Tβ4 and its fragments are found in blood, urine, tears, and wound fluid, and are known to act at distant sites from the source of secretion.

Why TB-500’s Systemic Reach Matters in Repair Research

Thymosin beta-4 is unusual among tissue repair mediators because it is a secreted intracellular protein with autocrine, paracrine, and endocrine activity. Following injury, Tβ4 levels rise locally and systemically, indicating a coordinated response. The actin-binding LKKTETQ motif promotes G-actin sequestration, which drives lamellipodia formation, endothelial cell migration, and tube formation — key steps in angiogenesis. TB-500 simultaneously downregulates NF-κB-driven inflammatory signaling and upregulates matrix metalloproteinases (MMPs) involved in extracellular matrix remodeling. Its systemic mobility — documented in wound exudate, plasma, and remote organ tissue after peripheral injury — distinguishes it from locally-acting growth factors and makes it a useful tool for studying coordinated multi-tissue repair responses.

Research Applications

TB-500 is used in studies examining:

• Wound healing models — dermal, corneal, and musculoskeletal tissue; actin-mediated cell migration quantification
• Angiogenesis — endothelial tube formation assays, VEGF crosstalk, capillary density measurement
• Tendon and ligament repair — collagen remodeling, fibroblast migration, MMP activity in connective tissue models
• Cardiac tissue research — myocardial infarction models, epicardial progenitor activation, ejection fraction recovery
• Anti-inflammatory pathway research — NF-κB inhibition, cytokine profile modulation in acute injury
• Actin biology — G-actin:F-actin ratio regulation, WH2 domain binding kinetics, cytoskeletal dynamics
• Systemic repair signaling — remote organ response to peripheral injury, Tβ4 as a circulating mediator

Specifications

Reconstitution

TB-500 arrives as lyophilized powder and must be reconstituted with bacteriostatic water prior to use. Use the formula:

Total mg ÷ Volume added (mL) = Concentration (mg/mL)

Example: 5mg vial + 1mL BAC water = 5mg/mL solution; 10mg vial + 2mL BAC water = 5mg/mL solution

Reconstituted peptide should be stored at 2–8°C and used within 28–30 days.

Protocol Notes

Animal model studies and early clinical literature provide the following reference frameworks — not instructions for use:

• Dose range: 2–2.5mg twice weekly in most rodent and equine wound healing studies; loading phases of 5mg/week followed by 2.5mg/week maintenance documented in preclinical literature
• Frequency: Twice weekly (loading) transitioning to once weekly (maintenance) in longer-duration studies
• Study duration: 4–8 weeks in musculoskeletal models; 2–4 weeks in acute wound and cardiac studies

Research Stacks

TB-4 (Thymosin Beta-4) is commonly paired in research settings with:

• BPC-157 — BPC-157 promotes angiogenesis and upregulates growth factor receptors in connective tissue; pairing with TB-4’s actin sequestration, cell migration facilitation, and anti-inflammatory properties provides researchers a model to study complementary regenerative mechanisms at sites of tissue disruption.
• GHK-Cu — GHK-Cu activates wound-healing gene programs and stimulates collagen and glycosaminoglycan synthesis; combined with TB-4’s cytoskeletal remodeling and stem cell recruitment activity, the pairing is studied in models of dermal and connective tissue repair.
• Ipamorelin — Ipamorelin stimulates pulsatile GH release via the ghrelin receptor, supporting IGF-1-mediated anabolic and tissue maintenance signaling; researchers pair with TB-4 to examine whether systemic GH axis activation augments the localized cell migration and repair mechanisms driven by thymosin peptides.

Purity Guarantee

Every batch is ≥99% purity. If you independently test your compound and the results don’t match — send us the COA and we’ll issue store credit, no questions asked.