NAD+

Volume Pricing

Price per pack (10 vials). Discount applies to this compound only – no mix and match.

NAD+ — Nicotinamide Adenine Dinucleotide / Sirtuin Coenzyme and Redox Research

Nicotinamide Adenine Dinucleotide (NAD+) is a coenzyme present in every living cell, functioning as the central electron carrier in oxidative metabolism and as a required substrate for a class of regulatory enzymes — sirtuins and PARPs — that govern DNA repair, gene silencing, and mitochondrial biogenesis. Interest in NAD+ as a research target intensified following work by David Sinclair at Harvard and Johan Auwerx at EPFL, whose publications between 2013 and 2016 demonstrated that age-associated NAD+ decline in mammals produces measurable deterioration in sirtuin-dependent processes, and that NAD+ restoration via precursors (NMN, NR) or direct supplementation partially reversed these deficits in aged rodents. Direct NAD+ research — particularly via intravenous delivery to bypass low oral bioavailability — allows investigators to study the immediate biochemical effects of NAD+ repletion without the metabolic conversion steps required by precursor approaches.

Why NAD+’s Dual Role as Redox Carrier and Signaling Substrate Matters

NAD+ operates in two distinct modes. In its redox role, it accepts electrons from metabolic intermediates (becoming NADH), driving ATP production in the electron transport chain. In its signaling role, NAD+ is consumed — not recycled — as a substrate by sirtuins (SIRT1, SIRT3, SIRT6), PARP enzymes, and CD38, meaning cellular NAD+ availability directly gates the activity of these regulatory proteins. SIRT1 and SIRT6 deacetylate histones and transcription factors involved in aging, inflammation, and circadian regulation; SIRT3 governs mitochondrial protein acetylation; PARP-1 drives DNA strand break repair. Declining NAD+ with age thus produces a compound deficit across all three systems simultaneously — a mechanistic rationale that has made NAD+ restoration one of the more intensively studied interventions in aging biology.

Research Applications

NAD+ is used in studies examining:

• Sirtuin pathway biology — SIRT1, SIRT3, SIRT6 activity as a function of NAD+ availability
• DNA damage response and PARP-mediated repair kinetics
• Aging biology — NAD+ decline curves, tissue-specific depletion, and restoration effects
• Mitochondrial biogenesis and oxidative phosphorylation efficiency
• Neurodegeneration models — NAD+ restoration in Alzheimer’s, Parkinson’s, and axonal degeneration research
• Metabolic disease models — insulin sensitivity, hepatic lipid metabolism, and adipose tissue biology
• Comparative bioavailability — direct NAD+ vs. NMN vs. NR precursor restoration kinetics

Specifications

Protocol Notes

Oral bioavailability of exogenous NAD+ is limited due to degradation in the gut prior to absorption; published clinical research has primarily used IV infusion for direct repletion studies. Dose ranges below reflect IV administration frameworks seen in the literature.

• Dose range: 500–1000mg IV infusion studied in human clinical research; lower ranges (250–500mg) in initial tolerability studies
• Frequency: Single-dose studies common; repeated infusion (daily or weekly) studied in longer-duration research
• Study duration: Acute pharmacokinetic studies (single session) through multi-week protocols in aging and metabolic research

Research Stacks

NAD+ is commonly paired in research settings with:

• MOTS-c — MOTS-c activates AMPK and regulates mitochondrial energy substrate utilization; pairing with exogenous NAD+ is studied to probe whether replenishing the obligate sirtuin/PARP coenzyme potentiates MOTS-c’s metabolic effects in aging and metabolic disease research models.
• SS-31 — SS-31 reduces mitochondrial membrane ROS and stabilizes the electron transport chain; combining it with NAD+ is studied to address mitochondrial dysfunction from two angles — substrate availability and oxidative stress — within the same model.
• Glutathione — Glutathione is the primary cellular antioxidant and depends on NADPH (derived from NAD+) for its regeneration cycle; researchers pair the two to study whether systemic NAD+ restoration improves glutathione recycling capacity and overall redox buffering in aging or metabolic stress models.

Purity Guarantee

Every batch is ≥99% purity. If you independently test your compound and the results don’t match — send us the COA and we’ll issue store credit, no questions asked.